ABSTRACT
Background: Genetic polymorphisms of drug metabolisms by cytochrome P450 [P450s] could affect drug response, attracting particular interest in the pharmacogenetics. Due to the importance of CYP2C19* 17 allele and its capability of super- fast metabolism and also lack of information about distribution of the alleles in Iranian population, this research aimed to use High Resolution Melting [HRM] method compared to PCR-RFLP for genotyping healthy Iranian population
Methods: Blood samples were collected from 100 healthy Iranian volunteers. DNA was extracted by salting out method. Real-time PCR was used for amplification of the CYP2C19 gene and the alleles were identified by HRM. Sequencing was used to confirm the amplified DNA fragments and data were analyzed using SPSS software ver.18
Results: The frequency of alleles CYP2C19*1/*1, CYP2C19*1/*17 and CYP2C19*17/*17 were estimated as 58.33, 29.1 and 11.1%, respectively. Specificity and sensitivity of HRM method were 90% and 100%, with respect to PCR-RFLP. Also, HRM analysis has been evaluated as a faster and more effective approach
Conclusion: Comparison of our results based on HRM analysis with PCR-RFLP showed that our developed method is rapid, accurate, fast and economic to study the CYP2C19*17 allele and it is appropriate for other similar population genetic studies
ABSTRACT
Objective: In order to improve the water solubility and bioavailability of curcumin in cancer therapy, we prepared and tested a novel waterborne cationic polyurethane [PU] as a nano-carrier for curcumin loading [CU-PU]. We studied the effect of this prepared nano-drug on melanoma [F10B16] and fibroblasts cells [L929]
Methods: Morphology, size and cell internalization ability of the prepared nanoparticles were analyzed by zetasizer, SEM, AFM and fluorescent microscopy, respectively. We anticipated that curcumin was loaded in the hydrophobic core of the PU carrier. Next, the suitable dose and therapeutic effects of CU-PU for both skin cancer and normal cell lines were evaluated by the MTT assay and real-time PCR
Results: The average diameters and polydispersity of the nanoparticles were 62.37 +/- 1.7 nm and 0.080 +/- 2.1 at 25 C, respectively. The drug encapsulation efficiency was 87 +/- 0.2%. The morphological analysis confirmed both a spherical shape and good dispersion without remarkable aggregation. The MTT assay results showed that the IC50 at 24 hours was 36.2 microM, whereas it was 25.4 microM at 48 hours. Real-time PCR results indicated that the CU-PU significantly decreased mRNA expressions of VEGF, Bcl-2, MMP-9 and COX-2 genes. An increase in mRNA expression of the BAX gene was also observed
Conclusion: Our result provided acceptable evidence for cell proliferation inhibition and the apoptotic effect of CU-PU on skin cancer cells. There were no adverse effects detected for normal cells
ABSTRACT
Herein, iron-gold core shell magnetic nanoparticles Fe[commercial at]Au NPs was investigated as contrasting agent in radiation therapy in the breast cancer. Assessment of cytotoxic and radio sensitizing potential was done by MTT method and Flow cytometry. Radiation was done using Co 60 source. The response of cells to treatment with radiation alone and radiation with nanoparticles was assessed. The study demonstrates that Fe[commercial at]Au nanoparticles do not have considerable cytotoxic effects, but they increase the effectiveness of radiation that means the survival of the group without nanoparticles exposed to 5 Gy radiations is 75%while the group with nanoparticles is 33%. With 2 Gy radiations the survival of the two groups are 87% and 80% respectively